• Abrupt permafrost thaw triggers activity of copiotrophs and microbiome predators 

      Scheel, Maria; Zervas, Athanasios; Rijkers, Ruud; Tveit, Alexander Tøsdal; Ekelund, Flemming; Campuzano Jiménez, Francisco; Christensen, Torben R.; Jacobsen, Carsten S. (Journal article; Tidsskriftartikkel; Peer reviewed, 2023-10-05)
      Permafrost soils store a substantial part of the global soil carbon and nitrogen. However, global warming causes abrupt erosion and gradual thaw, which make these stocks vulnerable to microbial decomposition into greenhouse gases. Here, we investigated the microbial response to abrupt in situ permafrost thaw. We sequenced the total RNA of a 1 m deep soil core consisting of up to 26 500-year-old permafrost ...
    • AgNO3 Sterilizes Grains of Barley (Hordeum vulgare) without Inhibiting Germination—A Necessary Tool for Plant–Microbiome Research 

      Munkager, Victoria; Vestergård, Mette; Primé, Anders; Altenburger, Andreas; de Visser, Eva; Johansen, Jesper Liengaard; Ekelund, Flemming (Journal article; Tidsskriftartikkel; Peer reviewed, 2020-03-17)
      To understand and manipulate the interactions between plants and microorganisms, sterile seeds are a necessity. The seed microbiome (inside and surface microorganisms) is unknown for most plant species and seed-borne microorganisms can persist and transfer to the seedling and rhizosphere, thereby obscuring the effects that purposely introduced microorganisms have on plants. This necessitates that ...
    • Can bryophyte groups increase functional resolution in tundra ecosystems? 

      Lett, Signe; Jonsdottir, Ingibjorg Svala; Cooper, Elisabeth; Becker-Scarpitta, Antoine; Christiansen, Casper T.; During, Heinjo; Ekelund, Flemming; Henry, Gregory H. R.; Lang, Simone I.; Michelsen, Anders; Rousk, Kathrin; Alatalo, Juha M.; Betway, Katlyn R.; Busca, Sara; Callaghan, Terry; Carbognani, Michele; Cornelissen, Hans C.; Dorrepaal, Ellen; Egelkraut, Dagmar; Elumeeva, Tatiana G.; Hollister, Robert D.; Jägerbrand, Annika K.; Keuper, Frida; Klanderud, Kari; Lévesque, Esther; Liu, Xin; May, Jeremy; Michel, Pascale; Mörsdorf, Martin; Petraglia, Alessandro; Rixen, Christian; Robroek, Bjorn J.M.; Rzepczynska, Agnieszka M.; Soudzilovskaia, Nadeja A.; Tolvanen, Anne; Vandvik, Vigdis; Volkov, Igor; Volkova, Irina; van Zuijlen, Kristel (Journal article; Tidsskriftartikkel; Peer reviewed, 2021-08-30)
      The relative contribution of bryophytes to plant diversity, primary productivity, and ecosystem functioning increases towards colder climates. Bryophytes respond to environmental changes at the species level, but because bryophyte species are relatively difficult to identify, they are often lumped into one functional group. Consequently, bryophyte function remains poorly resolved. Here, we explore ...
    • Can bryophyte groups increase functional resolution in tundra ecosystems? 

      Lett, Signe; Jónsdóttir, Ingibjörg Svala; Becker-Scarpitta, Antoine; Christiansen, Casper T.; During, Heinjo; Ekelund, Flemming; Henry, Greg H.R.; Lang, Simone; Michelsen, Anders; Rousk, Kathrin; Alatalo, Juha; Betway, Katlyn Rose; Busca, Sara; Callaghan, Terry; Carbognani, Michele; Cooper, Elisabeth J.; Cornelissen, J. Hans C.; Dorrepaal, Ellen; Egelkraut, Dagmar Dorothea; Elumeeva, Tatiana G.; Haugum, Siri Vatsø; Hollister, Robert D.; Jägerbrand, Annika K.; Keuper, Frida; Klanderud, Kari; Lévesque, Esther; Liu, Xin; May, Jeremy L.; Michel, Pascale; Mörsdorf, Martin; Petraglia, Alessandro; Rixen, Christian; Robroek, Bjorn J.M.; Rzepczynska, Agnieszka M.; Soudzilovskaia, Nadejda A.; Tolvanen, Anne; Vandvik, Vigdis; Volkov, Igor; Volkova, Irina; van Zuijlen, Kristel (Journal article; Tidsskriftartikkel; Peer reviewed, 2021-08-30)
      The relative contribution of bryophytes to plant diversity, primary productivity, and ecosystem functioning increases towards colder climates. Bryophytes respond to environmental changes at the species level, but because bryophyte species are relatively difficult to identify, they are often lumped into one functional group. Consequently, bryophyte function remains poorly resolved. Here, we explore ...
    • Inter-laboratory testing of the effect of DNA blocking reagent G2 on DNA extraction from low-biomass clay samples 

      Jacobsen, Carsten Suhr; Nielsen, Tue Kjærgaard; Vester, Jan Kjølhede; Stougaard, Peter; Nielsen, Jeppe Lund; Voriskova, Jana; Winding, Anne; Baldrian, Petr; Liu, Binbin; Frostegård, Åsa; Pedersen, Dorthe; Tveit, Alexander Tøsdal; Svenning, Mette Marianne; Tebbe, Christoph C.; Øvreås, Lise; Jakobsen, Pia Bach; Blazewicz, Steven J.; Hubablek, Valerie; Bertilsson, Stefan; Hansen, Lars Hestbjerg; Cary, S. Craig; Holben, William E.; Ekelund, Flemming; Bælum, Jacob (Journal article; Tidsskriftartikkel; Peer reviewed, 2018-04-09)
      Here we show that a commercial blocking reagent (G2) based on modified eukaryotic DNA significantly improved DNA extraction efficiency. We subjected G2 to an inter-laboratory testing, where DNA was extracted from the same clay subsoil using the same batch of kits. The inter-laboratory extraction campaign revealed large variation among the participating laboratories, but the reagent increased the ...
    • Soil microorganisms decrease barley biomass uniformly across contrasting nitrogen availability 

      Munkager, Victoria; Altenburger, Andreas; Priemé, Anders; Bang-Andreasen, Toke; Rønn, Regin; Vestergård, Mette; Ekelund, Flemming (Journal article; Tidsskriftartikkel; Peer reviewed, 2021-03-30)
      <i>Aims</i> - Microorganisms play a dichotomous role in the soil nitrogen cycle through mineralization and immobilization. We aimed to understand how nitrogen availability modifies the effect of microorganisms on plant growth. We hypothesized that soil microorganisms would increase plant biomass following amendment with a substrate rich in organic nitrogen (net mineralization), be neutral when adding ...